Parallel to the event, four courses will be held that you can access by paying registration (value not included in the price of the symposium) and three free courses that will be held on Tuesday, December 12 (registration closed).
6:00 p.m. - 7:30 p.m. Postgraduate Room Patricio Barriga Fac. Cs. Agricultural and Food

Formation of collections of microbial cultures

Contents: Organization of a collection; Ex situ preservation of microorganisms by cryopreservation and lyophilization; Distribution of microorganisms.

Jean Castro F.
6:00 p.m. - 7:30 p.m. Agronomy Room 2

Determination of morphological parameters of leaves, roots and seeds, through image analysis

Contents: Introduction to morphological analysis based on images; Imaging systems; Open and paid software for determining morphological parameters.

Pedro Montesano
6:00 p.m. - 7:30 p.m. Agronomy Room 3

Collection of seeds of wild species for conservation and restoration purposes

Contents: General aspects of seed collection; Planning a seed collection campaign; Species identification and seed collection techniques.

Carolina Pañitrur D.
4:30 p.m. - 6:00 p.m. Postgraduate Room Patricio Barriga Fac. Cs. Agricultural and Food

Molecular identification of microorganisms by multilocus

Contents: DNA extraction and sequencing of multiple genes; Alignment, concatenation and realization of phylogenetic trees; Creation of sequence databases.

Jorge Carrasco F.
9:00 a.m. - 12:30 p.m. Agronomy Room 2

Metabarcoding, metagenomics and metatranscriptomics: When, how and why?

In this workshop we will talk about how to redirect a mixed-sample HTS experiment to resolve specific questions that arise from such designs: What is the sequencing effort required? What is the appropriate read size? Do you need amplification sequencing? , shotgun sequencing or RNA? Should sequencing be Single Read or Paired End? Do we use ASV or OTUS in taxonomic assignment? Do we deplete ribosomal or enrich in mRNA? We will address these and other questions, and how to resolve them, reviewing some case studies in which I have participated as a researcher or in years of consulting in the AUSTRAL-omics laboratory.

Andrea Silva Baez
2:00 pm - 5:30 pm Agronomy Room 2

Bioinformatics Analysis of Metabarcoding: exploring microbial diversity using sequencing data

This short bioinformatic analysis of metabarcoding data workshop is designed to provide participants with the essential skills necessary to process, analyze and interpret sequencing data generated through the metabarcoding technique. Participants will explore the key concepts of metabarcoding and learn about the bioinformatics tools and workflows necessary to understand microbial diversity in environmental samples. Likewise, functional metagenomics and potential applications of metabarcoding will be presented. Prerequisites: Basic understanding of molecular biology and bioinformatics.

Suany Quesada Calderón
9:00 a.m. - 5:30 p.m. Postgraduate Room Patricio Barriga Fac. Cs. Agricultural and Food

Introduction to participatory plant breeding methods

Introduction to methods and tools necessary to conduct activities to improve seeds of traditional varieties grown by small farmers. Principles and importance of participatory improvement and its impacts. Methods for selection and participatory evaluation of varieties in the field. Methods for evaluating the quality of grain and/or seeds with farmers. Methods for participatory planning of activities in a participatory plant breeding project.

Activity supported by ANID, International Linkage FOVI220099

Alberto Chassaigne
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